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W. W. = 10000). By contrast, both EDTA and cytochalasin B increase the transport of all four hydrophilic markers and do so to a greater degree than Pz-peptide. On this basis it may be concluded that the three enhancers differ in the mechanism by which they enhance paracellular permeability. Specifically, Pz-peptide opens a smaller fraction of the tight junctions and opens a given tight junction to a smaller extent than does EDTA or cytochalasin B. Oral Route of Peptide and Protein Drug Delivery 43 In addition, Pz-peptide is reversible in action in the presence of 2 mM extracellular Ca2+, returning the transepithelial resistance of the epithelial cell monolayer to resting value within 10 min following 10 min exposure and within 50 min following 120 min exposure (Yen and Lee, unpublished data).
2A-C. Specificity and titer of antibodies against bromelain. Antibodies against bromelain were both investigated for specificity and titer against bromelain. AImmunoelectrophoresis of a sample of crude bromelain. Only two bands corresponding to the two bromelain isoenzymes used for immunizing rabbits are visible. B Double immunodifusion of antiserum against increasing dilutions of antigen (left), or increasing dilutions of antiserum against a fixed amount of bromelain (right). C Immunoelectrophoresis of a bromelain sample in human plasma.
Drug Targeting 1:29-39 42. Conradi RA, Hilgers AR, Ho NFH, Burton PS (1991) The influence of peptide structure on transport across CaCO-2 cells. Pharm Res 8:1453-1460 43. Cereijido M, Meza I, Martinez-Palomo A (1981) Occluding junctions in cultured epithelial monolayers. Am J Physio1240:C96-C102 44. Madara JL, Dharmsathaphorn K (1985) Occluding junction structure-function relationships in a cultured epithelial monolayer. J Cell BioI 101:2124--2133 45. Yen WC, Lee VHL (1991) Influence of collagenase on the intestinal transport of a pentapeptide.